Description_x000D_ General description_x000D_ Tropoelastin is a 60-72 kDa alternatively spliced extracellular matrix protein. It is a soluble precursor of elastin and is distributed in elastic tissues. Tropoelastin consists of an N- terminal 26-amino-acid signal peptide and highly basic strongly conserved C-terminal domain, that is required for both incorporation into elastin and for cellular interactions. It Is also characterized with a hydrophobic domain and exon 26A, which is an unusual highly hydrophilic domain._x000D_ Application_x000D_ Preparation Procedure_x000D_ Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice._x000D_ 1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg._x000D_ 2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy._x000D_ 3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 μg/ml._x000D_ 4. Add appropriate amount of diluted tropoelastin material to the culture surface._x000D_ 5. Incubate at room temperature or 37 °C, covered, for approximately 1.5 hours._x000D_ 6. After incubation, aspirate any remaining material._x000D_ 7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces._x000D_ 8. Coated surfaces are ready for use. They may also be stored at 2-10 °C damp or air dried if sterility is maintained._x000D_ Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques._x000D_ Biochem/physiol Actions_x000D_ Tropoelastin exhibits the property of coacervation and plays a vital role in fiber formation and assembly. Abnormality in the tropoelastin is associated with the development of hereditary disease, cutis laxa (where the elasticity of the skin is lost)._x000D_ Features and Benefits_x000D_ Tropoelastin available in its native state. Unique since it becomes cross-linked immediately after its synthesis by the cell and during its export into the extracellular matrix._x000D_ Other Notes_x000D_ Storage at -20 °C prior to solubilization is recommended. Storage at 2-10 °C after reconstitution is recommended._x000D_ Shelf life is 24 months when stored at -20 °C. Shelf life after reconstitution is 3 months when stored at 2-10 °C