Description_x000D_ General description_x000D_ Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts._x000D_ Application_x000D_ Yeast Nitrogen Base With Amino Acids has been used:_x000D_ • in the ZYP-31 media preparation for E.coli_x000D_ • in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source_x000D_ • to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard_x000D_ • in the preparation of imaging medium_x000D_ Packaging_x000D_ 250 g in poly bottle_x000D_ Components_x000D_ Component, amount (μg/L unless indicated)_x000D_ Nitrogen Sources:_x000D_ Ammonium sulfate, 5.0 g/L_x000D_ Amino Acids:_x000D_ L-histidine, 10 mg/L_x000D_ DL-methionine, 20 mg/L_x000D_ DL-tryptophan, 20 mg/L_x000D_ Vitamins:_x000D_ Biotin, 2.0_x000D_ Calcium pantothenate, 400_x000D_ Folic acid, 2.0_x000D_ Inositol, 2.0 mg/L_x000D_ Nicotinic acid, 400_x000D_ p-Aminobenzoic acid, 200_x000D_ Pyridoxine HCl, 400_x000D_ Riboflavin, 200_x000D_ Thiamine HCL, 400_x000D_ Trace Elements:_x000D_ Boric acid_x000D_ Copper sulfate_x000D_ Potassium iodide_x000D_ Ferric chloride_x000D_ Magnesium sulfate_x000D_ Sodium molybdate_x000D_ Zinc sulfate_x000D_ Salts:_x000D_ Potassium phosphate monobasic, 1.0 g/L_x000D_ Magnesium sulfate, 0.5 g/L_x000D_ Sodium chloride, 0.1 g/L_x000D_ Calcium chloride, 0.1 g/L_x000D_ Reconstitution_x000D_ Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C._x000D_ Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.